5XMY
Crystal structure of TAF3 PHD finger bound to H3K4me3Q5ser
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | AREA DETECTOR |
Collection date | 2015-03-17 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 26.982, 48.096, 52.700 |
Unit cell angles | 90.00, 98.16, 90.00 |
Refinement procedure
Resolution | 25.274 - 1.699 |
R-factor | 0.1835 |
Rwork | 0.182 |
R-free | 0.22060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5c13 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.915 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (dev_2689) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.730 |
High resolution limit [Å] | 1.699 | 4.610 | 1.700 |
Rmerge | 0.069 | 0.046 | 0.856 |
Number of reflections | 14658 | ||
<I/σ(I)> | 14.2 | ||
Completeness [%] | 99.2 | 96.9 | 99.4 |
Redundancy | 5.1 | 4.4 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.03 M magnesium chloride, 0.03 M calcium chloride, 0.1 M MES, 0.1 M imidazole, PH6.5, 15% PEGMME 550, 15% PEG 20K |