5XKO
Crystal structure of native Msmeg3575 deaminase from Mycobacterium smegmatis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-10-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.28 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 63.262, 108.335, 53.261 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.800 - 1.890 |
| R-factor | 0.141 |
| Rwork | 0.138 |
| R-free | 0.17800 |
| Structure solution method | SAD |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.872 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXCD |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.800 | 2.000 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Number of reflections | 29961 | |
| <I/σ(I)> | 73 | |
| Completeness [%] | 99.8 | 99.3 |
| Redundancy | 39.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2 M magnesium acetate tetrahydrate, 0.1 M sodium cacodylate tri-hydrate pH 6.0 and 15% PEG 8000 |
