5XJ0

T. thermophilus RNA polymerase holoenzyme bound with gp39 and gp76

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	90
Detector technology	PIXEL
Collection date	2015-10-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 32 2 1
Unit cell lengths	294.427, 294.427, 222.774
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.777 - 4.004
R-factor	0.2386
Rwork	0.237
R-free	0.27150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3wod
RMSD bond length	0.008
RMSD bond angle	1.343
Data reduction software	SCALEPACK
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	4.140
High resolution limit [Å]	4.000	8.600	4.000
Rmerge	0.202	0.051
Rmeas	0.213	0.054
Rpim	0.085	0.017	0.655
Total number of observations	890829
Number of reflections	93258
<I/σ(I)>	2.9
Completeness [%]	99.6	98.8	100
Redundancy	9.6	10	8.4
CC(1/2)		0.999	0.653

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	50% tacsimate