5XHG
Crystal structure of Trastuzumab Fab fragment bearing Ne-(o-azidobenzyloxycarbonyl)-L-lysine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-08-04 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.651, 79.891, 85.764 |
| Unit cell angles | 113.50, 92.82, 102.84 |
Refinement procedure
| Resolution | 25.438 - 1.760 |
| R-factor | 0.1735 |
| Rwork | 0.173 |
| R-free | 0.19940 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.812 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_1839) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 1.760 |
| Number of reflections | 230256 |
| <I/σ(I)> | 7.92 |
| Completeness [%] | 95.8 |
| Redundancy | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | Ammonium sulfate, PEG 3350, Bis-Tris |
