5XGM
Crystal structure of EGFR 696-1022 T790M in complex with Go6976
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-07-01 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97853 |
Spacegroup name | I 2 3 |
Unit cell lengths | 146.310, 146.310, 146.310 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.267 - 2.952 |
R-factor | 0.204 |
Rwork | 0.203 |
R-free | 0.23180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2jit |
RMSD bond length | 0.009 |
RMSD bond angle | 1.102 |
Data reduction software | DENZO |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 46.270 |
High resolution limit [Å] | 2.950 |
Number of reflections | 11044 |
<I/σ(I)> | 30.3 |
Completeness [%] | 100.0 |
Redundancy | 39 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 293 | 0.1M NaCl,0.05M Glycine pH 9.5, 39% PEG 300 |