5XEQ
Crystal Structure of human MDGA1 and human neuroligin-2 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97950 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 154.875, 69.134, 108.843 |
| Unit cell angles | 90.00, 115.31, 90.00 |
Refinement procedure
| Resolution | 49.199 - 3.136 |
| R-factor | 0.2385 |
| Rwork | 0.234 |
| R-free | 0.27790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bl8 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.680 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.200 | 49.200 | 3.350 |
| High resolution limit [Å] | 3.140 | 8.870 | 3.140 |
| Rmerge | 0.075 | 0.040 | 0.261 |
| Rmeas | 0.078 | 0.042 | 0.272 |
| Rpim | 0.022 | 0.013 | 0.075 |
| Total number of observations | 236647 | 10309 | 42385 |
| Number of reflections | 18485 | 3292 | |
| <I/σ(I)> | 22.9 | 46.1 | 8.4 |
| Completeness [%] | 100.0 | 99.4 | 100 |
| Redundancy | 12.8 | 11.8 | 12.9 |
| CC(1/2) | 0.991 | 0.978 | 0.993 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 296 | 300mM Ammonium citrate tribasic, pH 7.0, 13% 1,3-Butanediol and 18%(v/v) PEG3350 |
