5XEN
Crystal structure of a hydrogen sulfide-producing enzyme (Fn1220) from Fusobacterium nucleatum in complex with L-serine-PLP Schiff base
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2009-12-15 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 116.869, 116.869, 99.393 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.500 - 1.940 |
| R-factor | 0.16 |
| Rwork | 0.158 |
| R-free | 0.18600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.419 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.500 | 1.990 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.080 | 0.244 |
| Number of reflections | 51457 | |
| <I/σ(I)> | 38.4 | 16.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 27.4 | 27.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 41% (v/v) PEG 600, 0.1M Hepes pH 7.5, 0.17M Calcium acetate |
