5XEB
Structure of the envelope glycoprotein of Dhori virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97845 |
| Spacegroup name | P 3 |
| Unit cell lengths | 106.729, 106.729, 134.440 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.935 - 2.497 |
| R-factor | 0.2311 |
| Rwork | 0.231 |
| R-free | 0.25710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.706 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.490 | 2.500 |
| Rmerge | 0.150 | 2.113 |
| Number of reflections | 59514 | 5937 |
| <I/σ(I)> | 14.492 | 1.078 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 8.5 | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 291 | 6% v/v Ethylene glycol, 0.1 M Citric acid pH 3.5, 10% w/v Polyethylene glycol 6.000 |
