5XE2

Endoribonuclease from Mycobacterial species

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-06-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	C 2 2 21
Unit cell lengths	49.475, 65.548, 57.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.010
R-factor	0.2046
Rwork	0.204
R-free	0.22270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xe3
RMSD bond length	0.007
RMSD bond angle	1.182
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.900
High resolution limit [Å]	2.010	5.070	1.870
Rmerge	0.146	0.077	0.734
Rmeas	0.157	0.082	0.787
Rpim	0.054	0.029	0.273
Number of reflections	7261
<I/σ(I)>	5.1
Completeness [%]	97.5	97.6	92.5
Redundancy	7.9	7.7	7.7
CC(1/2)		0.994	0.884

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7	293	0.2M sodium malonate at pH 7.0 and 20% (w/v) polyethylene glycol 3350