5XE2
Endoribonuclease from Mycobacterial species
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-06-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9795 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 49.475, 65.548, 57.390 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.010 |
R-factor | 0.2046 |
Rwork | 0.204 |
R-free | 0.22270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5xe3 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.182 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 1.900 |
High resolution limit [Å] | 2.010 | 5.070 | 1.870 |
Rmerge | 0.146 | 0.077 | 0.734 |
Rmeas | 0.157 | 0.082 | 0.787 |
Rpim | 0.054 | 0.029 | 0.273 |
Number of reflections | 7261 | ||
<I/σ(I)> | 5.1 | ||
Completeness [%] | 97.5 | 97.6 | 92.5 |
Redundancy | 7.9 | 7.7 | 7.7 |
CC(1/2) | 0.994 | 0.884 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 7 | 293 | 0.2M sodium malonate at pH 7.0 and 20% (w/v) polyethylene glycol 3350 |