5XDR
Crystal structure of human DEAH-box RNA helicase DHX15 in complex with ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-04 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 81.919, 89.377, 211.078 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 105.540 - 2.000 |
R-factor | 0.22523 |
Rwork | 0.223 |
R-free | 0.26911 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.656 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 105.900 |
High resolution limit [Å] | 2.000 |
Number of reflections | 31906 |
<I/σ(I)> | 18.4 |
Completeness [%] | 100.0 |
Redundancy | 13.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG4000, lithium sulfate, sodium acetate |