5XDF
Homoserine dehydrogenase from Thermus thermophilus HB8 complexed with HSE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-06-15 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 119.625, 119.625, 145.020 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.000 |
R-factor | 0.18634 |
Rwork | 0.185 |
R-free | 0.21913 |
RMSD bond length | 0.024 |
RMSD bond angle | 2.137 |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 80859 | 4013 |
<I/σ(I)> | 42 | 5.7 |
Completeness [%] | 99.8 | 100 |
Redundancy | 11 | 10.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 10 | 298 | Sodium formate, CAPS pH 10.0 |