5XAF
Crystal structure of tubulin-stathmin-TTL-Compound Z1 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-10-17 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9785 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 104.568, 156.509, 182.078 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.767 - 2.551 |
R-factor | 0.2075 |
Rwork | 0.206 |
R-free | 0.25690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4o2b |
RMSD bond length | 0.008 |
RMSD bond angle | 1.025 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 49.767 |
High resolution limit [Å] | 2.551 |
Number of reflections | 97756 |
<I/σ(I)> | 18.72 |
Completeness [%] | 100.0 |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 6% PEG 4000, 8% glycerol, 100mM MES, 30mM CaCl2, 30mM MgCl2, pH 6.7 |