5XA8
Complete structure factors and an atomic model of the calcium pump (SERCA1A) and associated phospholipids in the E1-ALF4-ADP-2CA2+ crystals
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-07-13 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 0.80 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 162.970, 75.020, 152.240 |
| Unit cell angles | 90.00, 109.31, 90.00 |
Refinement procedure
| Resolution | 79.640 - 3.200 |
| R-factor | 0.2146 |
| Rwork | 0.213 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1WPE |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.564 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 143.680 |
| High resolution limit [Å] | 3.200 |
| Number of reflections | 28956 |
| <I/σ(I)> | 40.29 |
| Completeness [%] | 100.0 |
| Redundancy | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 6.1 | 283 | PEG 400 |
