5X9Z
Crystal structure of inositol 1,4,5-trisphosphate receptor large cytosolic domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-18 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 212.503, 221.726, 318.452 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.145 - 7.311 |
| R-factor | 0.3209 |
| Rwork | 0.317 |
| R-free | 0.39370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | fragments of 3JAV C-alpha model and 3UJ4 chain A |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.452 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.100 | 7.572 |
| High resolution limit [Å] | 7.300 | 7.311 |
| Rmerge | 0.200 | 0.575 |
| Number of reflections | 10669 | 1030 |
| <I/σ(I)> | 10.5 | 2.31 |
| Completeness [%] | 99.9 | 99 |
| Redundancy | 11.3 | 8.1 |
| CC(1/2) | 0.993 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | sodium citrate |
