5X7N

Crystal structure of meso-diaminopimelate decarboxylase (DAPDC) from Corynebacterium glutamicum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-19
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97934
Spacegroup name	P 21 21 2
Unit cell lengths	114.543, 91.702, 95.161
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.461 - 1.720
R-factor	0.1725
Rwork	0.171
R-free	0.21120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5x7m
RMSD bond length	0.022
RMSD bond angle	2.124
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	HKL-2000
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	95.170	1.750
High resolution limit [Å]	1.720	1.720
Number of reflections	95643
<I/σ(I)>	40.23
Completeness [%]	95.2
Redundancy	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Sodium citrate tribasic, Sodium cacodylate