5X6Z
Crystal structure of Rice Dwarf Virus P5 in complex with GTP and GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-04-26 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 |
| Unit cell lengths | 77.394, 83.447, 141.979 |
| Unit cell angles | 103.10, 101.98, 95.69 |
Refinement procedure
| Resolution | 37.659 - 2.100 |
| R-factor | 0.1869 |
| Rwork | 0.184 |
| R-free | 0.23490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5x6x |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.882 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((dev_2614: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.069 | 0.437 |
| Number of reflections | 193037 | 9561 |
| <I/σ(I)> | 19.2 | 3.4 |
| Completeness [%] | 98.6 | 97.7 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 16% PEG 3350, 0.1M MES, pH 6.0 |
