5X6Y
Crystal structure of Rice Dwarf Virus P5 in complex with S-adenosylmethionine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-23 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 |
| Unit cell lengths | 60.182, 122.778, 129.257 |
| Unit cell angles | 73.80, 87.14, 86.08 |
Refinement procedure
| Resolution | 38.226 - 2.100 |
| R-factor | 0.2041 |
| Rwork | 0.202 |
| R-free | 0.24520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5x6x |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.908 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((dev_2614: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.040 | 0.389 |
| Number of reflections | 198192 | 9820 |
| <I/σ(I)> | 24 | 2.1 |
| Completeness [%] | 96.0 | 95 |
| Redundancy | 2.1 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 16% PEG 3350, 0.03M sodium citrate, 0.07M bis-tris propane, 0.2M sodium chloride, 0.01M tris(2-carboxyethyl)phosphine, 5mM S-adenosylmethionine, 1mM GpppG, 5mM magnesium chloride |
