5X6F
Crystal structure of Phosphopantetheine adenylyltransferase from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU ULTRAX 18 |
| Temperature [K] | 98.15 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-01-15 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 112.691, 65.035, 124.984 |
| Unit cell angles | 90.00, 101.25, 90.00 |
Refinement procedure
| Resolution | 32.388 - 2.593 |
| R-factor | 0.2673 |
| Rwork | 0.265 |
| R-free | 0.29060 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.583 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 32.390 |
| High resolution limit [Å] | 2.590 |
| Number of reflections | 29671 |
| <I/σ(I)> | 16.14 |
| Completeness [%] | 95.0 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 295 | PEG 4000, 0.1M HEPES pH 7.0, 5% isopropanol, 200mM sodium acetate |
