5X5V
Crystal structure of pseudorabies virus glycoprotein D
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-03-16 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.979 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 79.398, 60.975, 60.681 |
Unit cell angles | 90.00, 111.22, 90.00 |
Refinement procedure
Resolution | 23.531 - 1.500 |
R-factor | 0.1703 |
Rwork | 0.169 |
R-free | 0.19230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2c36 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.090 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Rpim | 0.129 | |
Number of reflections | 42212 | |
<I/σ(I)> | 25.5 | |
Completeness [%] | 97.6 | |
Redundancy | 6.1 | |
CC(1/2) | 0.966 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 0.1 M ammonium acetate 0.1 M bis-tris pH 5.5 17 % PEG 10,000 |