5X50
RNA Polymerase II from Komagataella Pastoris (Type-2 crystal)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-10-28 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0 |
Spacegroup name | P 31 |
Unit cell lengths | 211.750, 211.750, 132.280 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.704 - 4.293 |
R-factor | 0.238 |
Rwork | 0.235 |
R-free | 0.29570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vum |
RMSD bond length | 0.006 |
RMSD bond angle | 1.696 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.704 | 40.704 | 4.550 |
High resolution limit [Å] | 4.290 | 12.500 | 4.290 |
Rmerge | 0.188 | 0.057 | 1.479 |
Rmeas | 0.211 | 0.064 | 1.662 |
Total number of observations | 219141 | ||
Number of reflections | 45119 | 1779 | 7130 |
<I/σ(I)> | 5.97 | 23.31 | 0.98 |
Completeness [%] | 99.6 | 97.6 | 97.9 |
Redundancy | 4.857 | 4.395 | 4.766 |
CC(1/2) | 0.994 | 0.994 | 0.457 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 287 | 0.2 M sodium citrate, 8% PGA-LM and 1 % dextran sulfate sodium salt |