5X50

RNA Polymerase II from Komagataella Pastoris (Type-2 crystal)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2011-10-28
Detector	RAYONIX MX225HE
Wavelength(s)	1.0
Spacegroup name	P 31
Unit cell lengths	211.750, 211.750, 132.280
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.704 - 4.293
R-factor	0.238
Rwork	0.235
R-free	0.29570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vum
RMSD bond length	0.006
RMSD bond angle	1.696
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.704	40.704	4.550
High resolution limit [Å]	4.290	12.500	4.290
Rmerge	0.188	0.057	1.479
Rmeas	0.211	0.064	1.662
Total number of observations	219141
Number of reflections	45119	1779	7130
<I/σ(I)>	5.97	23.31	0.98
Completeness [%]	99.6	97.6	97.9
Redundancy	4.857	4.395	4.766
CC(1/2)	0.994	0.994	0.457

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		287	0.2 M sodium citrate, 8% PGA-LM and 1 % dextran sulfate sodium salt