5X4Z

RNA Polymerase II from Komagataella Pastoris (Type-1 crystal)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-05
Detector	RAYONIX MX225HE
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	155.048, 160.351, 254.354
Unit cell angles	90.00, 105.43, 90.00

Refinement procedure

Resolution	49.820 - 7.800
R-factor	0.3533
Rwork	0.353
R-free	0.35710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.819
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (dev_2614)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	8.080
High resolution limit [Å]	7.800	16.620	7.800
Rmerge	0.378	0.198
Rmeas	0.407	0.234
Rpim	0.270	0.123	0.838
Total number of observations	51715
Number of reflections	13973
<I/σ(I)>	2.2
Completeness [%]	99.9	99.8	100
Redundancy	3.7	3.5	3.8
CC(1/2)		0.961	0.423

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M sodium citrate, 8% PGA-LM, 10% glycerol