5X33

Leukotriene B4 receptor BLT1 in complex with BIIL260

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-11
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1
Spacegroup name	P 2 21 21
Unit cell lengths	69.560, 77.620, 135.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.539 - 3.700
R-factor	0.2508
Rwork	0.248
R-free	0.29580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4MBS and 4K5Y
RMSD bond length	0.005
RMSD bond angle	0.801
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.500	3.920
High resolution limit [Å]	3.700	3.700
Number of reflections	8291
<I/σ(I)>	6.7	1.1
Completeness [%]	99.9	100
Redundancy	34.6	31.8
CC(1/2)	0.967	0.535

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		277	PEG 300, Bicine