5X2Q
Crystal structure of the medaka fish taste receptor T1r2a-T1r3 ligand binding domains in complex with glycine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-01-29 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 98.517, 112.724, 128.685 |
Unit cell angles | 90.00, 92.16, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.640 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 84819 | 4175 |
<I/σ(I)> | 10.7 | 1.8 |
Completeness [%] | 98.0 | 97.2 |
Redundancy | 3 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 12~13% (w/v) PEG 1500, 3% (v/v) PEG400, 0.1 M MES |