5X1M
Vanillate/3-O-methylgallate O-demethylase, LigM, protocatechuate-tetrahydrofolate complex form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
Synchrotron site | Photon Factory |
Beamline | AR-NE3A |
Temperature [K] | 95 |
Detector technology | PIXEL |
Collection date | 2015-12-19 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 103.802, 118.370, 132.329 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.533 - 1.900 |
R-factor | 0.2099 |
Rwork | 0.208 |
R-free | 0.24230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5x1i |
RMSD bond length | 0.006 |
RMSD bond angle | 0.897 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 48.320 |
High resolution limit [Å] | 1.900 |
Number of reflections | 257350 |
<I/σ(I)> | 14.4 |
Completeness [%] | 100.0 |
Redundancy | 13.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M Tris pH 8.5, 0.1M LiCl, 25%(w/v) PEG8000 |