5X0Y
Complex of Snf2-Nucleosome complex with Snf2 bound to SHL2 of the nucleosome
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.69 |
| Software | e2boxer.py,RELION,UCSFImage,CTFFIND,PHENIX |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
