5WYZ
Crystal structure of human TLR8 in complex with CU-CPT9b
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2016-11-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 143.620, 98.950, 139.520 |
| Unit cell angles | 90.00, 108.39, 90.00 |
Refinement procedure
| Resolution | 132.390 - 2.300 |
| R-factor | 0.21102 |
| Rwork | 0.208 |
| R-free | 0.26004 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.675 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 132.390 |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 78179 |
| <I/σ(I)> | 12.3 |
| Completeness [%] | 100.0 |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG 4000, calcium chloride, Tris-HCl, ethylene glycol |
