5WYM

Crystal structure of an anti-connexin26 scFv

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	CMOS
Collection date	2016-03-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	P 4 21 2
Unit cell lengths	119.489, 119.489, 94.106
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.010 - 2.650
R-factor	0.237
Rwork	0.236
R-free	0.26320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ghw
RMSD bond length	0.007
RMSD bond angle	1.116
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.010	7.190	2.700
High resolution limit [Å]	2.650	5.710	2.650
Rmerge	0.101	0.073	0.367
Rmeas	0.108	0.078	0.429
Rpim	0.038	0.025	0.210
Number of reflections	19583
<I/σ(I)>	5.4
Completeness [%]	96.1	99	92.4
Redundancy	6.6	8.6	4
CC(1/2)		0.996	0.001

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M HEPES, 3 M NaCl