5WY9
Apo form crystal structure of human Lipocalin PGDS .
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-03 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.000030 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.270, 56.500, 72.529 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.570 - 1.450 |
| R-factor | 0.17809 |
| Rwork | 0.176 |
| R-free | 0.21090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4imn |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.521 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.500 | 1.470 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.039 | 0.465 |
| Number of reflections | 26866 | 1176 |
| <I/σ(I)> | 2.13 | 2.1 |
| Completeness [%] | 99.9 | 91.5 |
| Redundancy | 4.8 | 3 |
| CC(1/2) | 0.999 | 0.372 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 0.05M Potassium thiocyanate, 30%PEG MME 2000 |
