5WY6

Crystal structure of AtNUDX1 (E56A)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-09-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9785
Spacegroup name	C 2 2 21
Unit cell lengths	84.658, 117.306, 68.937
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.672 - 1.779
R-factor	0.1968
Rwork	0.196
R-free	0.21320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wwd
RMSD bond length	0.006
RMSD bond angle	0.769
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall
Low resolution limit [Å]	45.000
High resolution limit [Å]	1.668
Number of reflections	244523
<I/σ(I)>	18.1
Completeness [%]	99.4
Redundancy	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	Sodium acetate trihydrate, PEG 2000, Ammonium sulfate