5WXH
Crystal structure of TAF3 PHD finger bound to H3K4me3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-01-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 |
| Unit cell lengths | 27.244, 35.138, 38.562 |
| Unit cell angles | 82.96, 75.62, 73.49 |
Refinement procedure
| Resolution | 37.296 - 1.297 |
| R-factor | 0.1541 |
| Rwork | 0.153 |
| R-free | 0.17510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.860 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.320 |
| High resolution limit [Å] | 1.297 | 3.530 | 1.300 |
| Rmerge | 0.071 | 0.060 | 0.521 |
| Number of reflections | 31171 | ||
| <I/σ(I)> | 15.7 | ||
| Completeness [%] | 95.5 | 98.9 | 93.4 |
| Redundancy | 5.1 | 6.3 | 4.3 |
| CC(1/2) | 0.995 | 0.897 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.03 M magnesium chloride, 0.03 M calcium chloride, 0.1 M MES, 0.1 M imidazole, PH6.5, 15% PEGMME 550, 15% PEG 20K |
