5WVO

Crystal structure of DNMT1 RFTS domain in complex with K18/K23 mono-ubiquitylated histone H3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	93
Detector technology	PIXEL
Collection date	2016-11-08
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.1
Spacegroup name	C 2 2 21
Unit cell lengths	68.362, 198.779, 76.904
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.181 - 1.997
R-factor	0.198
Rwork	0.196
R-free	0.23870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ubq 3epz
RMSD bond length	0.019
RMSD bond angle	0.934
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.181	2.030
High resolution limit [Å]	1.997	2.000
Rmerge		0.706
Number of reflections	35892
<I/σ(I)>	6.7	3.6
Completeness [%]	99.8	99.1
Redundancy	6.4	6.1
CC(1/2)		0.778

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277	100mM Bis-Tris (pH 6.0), 200mM lithium sulfate monohydrate, 20% PEG 10000