5WVD
Structure of Mnk1 in complex with DS12881479
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2011-11-22 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 92.635, 92.635, 174.437 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.000 |
R-factor | 0.24215 |
Rwork | 0.239 |
R-free | 0.27121 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2hw6 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.534 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 15900 | |
<I/σ(I)> | 18.6 | 3.4 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.1 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 277 | polyethylene glycol 3350, ammonium sulfate, citrate buffer (pH 6.2), MgCl2 |