5WUP
Crystal structure of a insect group III chitinase (CAD1) from Ostrinia furnacalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 71.835, 71.835, 193.290 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.963 - 2.300 |
| R-factor | 0.1897 |
| Rwork | 0.188 |
| R-free | 0.21680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ybt |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.109 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11_2567: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.963 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 23352 | |
| <I/σ(I)> | 17.18 | |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 10.6 | 12.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 200 mM ammonium sulfate, 100 mM bis-Tris, 20% PEG3350 |
