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5WTP

Crystal structure of the C-terminal domain of outer membrane protein A (OmpA) from Capnocytophaga gingivalis

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeROTATING ANODE
Source detailsRIGAKU MICROMAX-007 HF
Temperature [K]100
Detector technologyIMAGE PLATE
Collection date2015-06-08
DetectorRIGAKU RAXIS IV++
Wavelength(s)1.54178
Spacegroup nameP 4 21 2
Unit cell lengths130.366, 130.366, 56.531
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution48.191 - 2.150
R-factor0.1844
Rwork0.182
R-free0.23320
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)3fby
RMSD bond length0.003
RMSD bond angle0.708
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwarePHENIX
Refinement softwarePHENIX (1.9_1692)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]48.1912.190
High resolution limit [Å]2.1502.150
Number of reflections26492
<I/σ(I)>7.1
Completeness [%]97.6
Redundancy5.1
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP8289100 mM Tris-HCl, pH 8.0, 3 M (NH4)2SO4

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