5WTJ

Crystal structure of an endonuclease

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-04
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9785
Spacegroup name	P 21 21 21
Unit cell lengths	91.658, 94.231, 338.218
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.375 - 3.503
R-factor	0.264
Rwork	0.263
R-free	0.29070
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.421
Data scaling software	HKL-3000
Phasing software	PHENIX (1.8.2_1309)
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.500
Rmerge	0.090	0.601
Number of reflections	30634
<I/σ(I)>	12.6
Completeness [%]	97.9
Redundancy	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.4M L-Proline, 0.1M HEPES pH 7.5, 10% PEG 3350