5WSK
Structure of Ribulose-1,5-bisphosphate carboxylase/oxygenase from wheat
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2013-11-26 |
Detector | RAYONIX MX225-HS |
Wavelength(s) | 0.979 |
Spacegroup name | P 4 |
Unit cell lengths | 110.720, 110.720, 200.956 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.415 - 1.781 |
R-factor | 0.1562 |
Rwork | 0.154 |
R-free | 0.18740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1uzh |
RMSD bond length | 0.012 |
RMSD bond angle | 1.333 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 44.415 |
High resolution limit [Å] | 1.780 |
Number of reflections | 226185 |
<I/σ(I)> | 1.34 |
Completeness [%] | 98.4 |
Redundancy | 1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291.15 | 0.1M Tris-HCl, pH 8.5, 12.5% PEG 3350 |