5WRO
Crystal structure of Drosophila enolase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97915 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 118.820, 118.820, 229.792 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.327 - 2.015 |
| R-factor | 0.1611 |
| Rwork | 0.159 |
| R-free | 0.19980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3b97 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.802 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.015 | 2.100 |
| Rmerge | 0.104 | 0.604 |
| Number of reflections | 41492 | |
| <I/σ(I)> | 12.7 | 2.1 |
| Completeness [%] | 98.0 | 99.7 |
| Redundancy | 3.2 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 289 | 10 mM CoCl2, 0.1 M CdCl2, 0.1 M MES pH6.5, 1.9 M (NH4)2SO4 |
