5WQ4
Crystal structure of OPTN and linear diubiquitin complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 298.15 |
| Detector technology | CCD |
| Collection date | 2014-11-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 43 |
| Unit cell lengths | 77.659, 77.659, 166.552 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.054 - 3.000 |
| R-factor | 0.2477 |
| Rwork | 0.245 |
| R-free | 0.30340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zvo |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.403 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.054 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 19725 | |
| <I/σ(I)> | 20.03 | |
| Completeness [%] | 100.0 | |
| Redundancy | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289.15 | 30% w/v PEG 3350, 0.1 M HEPES (pH 7.5) |
