5WPM
KRas G12V, bound to GppNHp and miniprotein 225-11(A30R)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.934, 37.810, 70.179 |
| Unit cell angles | 90.00, 103.38, 90.00 |
Refinement procedure
| Resolution | 68.280 - 1.720 |
| R-factor | 0.2372 |
| Rwork | 0.235 |
| R-free | 0.28510 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.161 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 68.300 |
| High resolution limit [Å] | 1.720 |
| Number of reflections | 27343 |
| <I/σ(I)> | 9.2 |
| Completeness [%] | 98.9 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M calcium chloride and 20% PEG3350 |
