5WOB

Crystal Structure Analysis of Fab1-Bound Human Insulin Degrading Enzyme (IDE) in Complex with Insulin

Replaces:  4Q5Z

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	121.589, 138.190, 376.509
Unit cell angles	90.00, 99.36, 90.00

Refinement procedure

Resolution	49.543 - 3.950
R-factor	0.2439
Rwork	0.243
R-free	0.29100
Starting model (for MR)	4iof
RMSD bond length	0.002
RMSD bond angle	0.453
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	4.020
High resolution limit [Å]	3.950	3.950
Rmerge	0.200	0.672
Rpim	0.130	0.424
Number of reflections	108370	5406
<I/σ(I)>	7	2.1
Completeness [%]	99.5	99.8
Redundancy	3.3	3.3
CC(1/2)		0.583

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291.15	0.1M Sodium cacodylate, pH6.5; 0.2M MgCl2; 10% PEG3000, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K