5WO8

Crystal Structure of Transient Receptor Potential (TRP) channel TRPV6*-Del1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-12
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9791
Spacegroup name	P 4 21 2
Unit cell lengths	145.317, 145.317, 113.699
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.744 - 3.400
R-factor	0.2831
Rwork	0.281
R-free	0.31550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5iwp
RMSD bond length	0.002
RMSD bond angle	0.604
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.400
Number of reflections	17211
<I/σ(I)>	1.22
Completeness [%]	99.2
Redundancy	8.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	150 mM nacl, 100 mM TRIS, 22% PEG 350