5WNO
Crystal structure of C. elegans LET-23 kinase domain complexed with AMP-PNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-04-30 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.11587 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.097, 77.978, 106.207 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.936 - 2.386 |
R-factor | 0.217 |
Rwork | 0.215 |
R-free | 0.24750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4rix |
RMSD bond length | 0.002 |
RMSD bond angle | 0.455 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 63.000 | 2.460 |
High resolution limit [Å] | 2.386 | 2.386 |
Rmerge | 0.100 | 0.890 |
Number of reflections | 16320 | |
<I/σ(I)> | 10.8 | 1.1 |
Completeness [%] | 98.8 | 98.5 |
Redundancy | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20% PEG5000 MME, 0.20 M sodium chloride, 0.10 M HEPES, pH 7.5 |