5WNA

Structure of antibody 3D3 bound to the central conserved region of RSV G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.11503
Spacegroup name	P 1 21 1
Unit cell lengths	64.620, 135.010, 73.780
Unit cell angles	90.00, 107.45, 90.00

Refinement procedure

Resolution	48.719 - 2.400
R-factor	0.1938
Rwork	0.192
R-free	0.24550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5i30
RMSD bond length	0.006
RMSD bond angle	0.935
Data reduction software	iMOSFLM
Data scaling software	Aimless (0.5.31)
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.720	48.720	2.480
High resolution limit [Å]	2.400	9.300	2.400
Rmerge	0.116	0.035	0.844
Rmeas	0.126	0.038	0.931
Rpim	0.050	0.014	0.386
Number of reflections	46906	826	4617
<I/σ(I)>	12.3
Completeness [%]	99.5	99.3	99
Redundancy	6.3	7.1	5.6
CC(1/2)	0.996	0.999	0.670

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295.15	23% PEG 3350, 0.05 M zinc acetate