5I30
Crystal structure of the human astrovirus 2 neutralizing monoclonal antibody PL-2 Fab fragment at 1.9 A resolution
Summary for 5I30
| Entry DOI | 10.2210/pdb5i30/pdb |
| Descriptor | Fab PL-2 light chain, Fab PL-2 heavy chain, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (4 entities in total) |
| Functional Keywords | antibody fab fragment, immune system |
| Biological source | Mus musculus More |
| Total number of polymer chains | 2 |
| Total formula weight | 47475.59 |
| Authors | Bogdanoff, W.A.,DuBois, R.M. (deposition date: 2016-02-09, release date: 2016-04-13, Last modification date: 2024-10-23) |
| Primary citation | Bogdanoff, W.A.,Morgenstern, D.,Bern, M.,Ueberheide, B.M.,Sanchez-Fauquier, A.,DuBois, R.M. De Novo Sequencing and Resurrection of a Human Astrovirus-Neutralizing Antibody. Acs Infect Dis., 2:313-321, 2016 Cited by PubMed Abstract: Monoclonal antibody (mAb) therapeutics targeting cancer, autoimmune diseases, inflammatory diseases, and infectious diseases are growing exponentially. Although numerous panels of mAbs targeting infectious disease agents have been developed, their progression into clinically useful mAbs is often hindered by the lack of sequence information and/or loss of hybridoma cells that produce them. Here we combine the power of crystallography and mass spectrometry to determine the amino acid sequence and glycosylation modification of the Fab fragment of a potent human astrovirus-neutralizing mAb. We used this information to engineer a recombinant antibody single-chain variable fragment that has the same specificity as the parent monoclonal antibody to bind to the astrovirus capsid protein. This antibody can now potentially be developed as a therapeutic and diagnostic agent. PubMed: 27213181DOI: 10.1021/acsinfecdis.6b00026 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.9 Å) |
Structure validation
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