5WMH
Arabidopsis thaliana prephenate aminotransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2015-07-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.988 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.343, 111.575, 141.775 |
| Unit cell angles | 90.00, 90.37, 90.00 |
Refinement procedure
| Resolution | 33.268 - 3.000 |
| R-factor | 0.2128 |
| Rwork | 0.210 |
| R-free | 0.27040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j32 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.925 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.270 | 3.050 |
| High resolution limit [Å] | 2.960 | 2.960 |
| Rmerge | 0.110 | 0.265 |
| Number of reflections | 51962 | 4284 |
| <I/σ(I)> | 15.43 | 7.8 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 1.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 20% (w/v) PEG-3350 and 200 mM magnesium formate |
