5WMF
Crystal structure of the Hexameric Ring of Epstein-Barr Virus Nuclear Antigen-1, EBNA1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-07 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 |
| Unit cell lengths | 31.280, 84.768, 84.709 |
| Unit cell angles | 60.01, 87.31, 88.53 |
Refinement procedure
| Resolution | 29.189 - 1.900 |
| R-factor | 0.2015 |
| Rwork | 0.198 |
| R-free | 0.27640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vhi |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.599 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 48887 |
| <I/σ(I)> | 9.68 |
| Completeness [%] | 82.4 |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | Concentrated EBNA1 was diluted 1:1 in a solution containing 50 mM morpholineethanesulfonic acid (MES) (pH 6.5) and 800 mM sodium formate. |
