5WLH
Crystal structure of LbaCas13a H328A (C2c2) bound to pre-crRNA (24-nt spacer)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-18 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.1158 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 150.842, 92.213, 115.858 |
| Unit cell angles | 90.00, 92.42, 90.00 |
Refinement procedure
| Resolution | 47.116 - 1.800 |
| R-factor | 0.185842735198 |
| Rwork | 0.184 |
| R-free | 0.21732 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5wih |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.827 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.32) |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.116 | 116.590 | 1.740 |
| High resolution limit [Å] | 1.710 | 9.360 | 1.710 |
| Rmerge | 0.051 | 0.028 | 1.174 |
| Rmeas | 0.057 | 0.031 | 1.325 |
| Rpim | 0.025 | 0.014 | 0.603 |
| Total number of observations | 807018 | 5538 | 21467 |
| Number of reflections | 162397 | 1114 | 4859 |
| <I/σ(I)> | 15.2 | 55 | 1.2 |
| Completeness [%] | 93.3 | 99.1 | 56.8 |
| Redundancy | 5 | 5 | 4.4 |
| CC(1/2) | 0.999 | 0.999 | 0.502 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.2 M ammonium iodide, 20 % PEG 3,350 |
