5WIT

Crystal structure of the Thermus thermophilus 70S ribosome in complex with pikromycin and bound to mRNA and A-, P- and E-site tRNAs at 2.6A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-26
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97950
Spacegroup name	P 21 21 21
Unit cell lengths	209.340, 447.210, 620.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	152.820 - 2.600
R-factor	0.2407
Rwork	0.238
R-free	0.28330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4y4p
RMSD bond length	0.004
RMSD bond angle	0.838
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	152.820	152.820	2.670
High resolution limit [Å]	2.600	11.630	2.600
Rmerge	0.139	0.023	1.221
Rmeas	0.165	0.028	1.459
Total number of observations	5786516
Number of reflections	1729501	19535	125032
<I/σ(I)>	8.58	35.37	0.97
Completeness [%]	98.3	94.1	96.4
Redundancy	3.346	3.286	3.191
CC(1/2)	0.991	0.998	0.173

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME