5WIT
Crystal structure of the Thermus thermophilus 70S ribosome in complex with pikromycin and bound to mRNA and A-, P- and E-site tRNAs at 2.6A resolution
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-03-26 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97950 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 209.340, 447.210, 620.610 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 152.820 - 2.600 |
R-factor | 0.2407 |
Rwork | 0.238 |
R-free | 0.28330 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4y4p |
RMSD bond length | 0.004 |
RMSD bond angle | 0.838 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 152.820 | 152.820 | 2.670 |
High resolution limit [Å] | 2.600 | 11.630 | 2.600 |
Rmerge | 0.139 | 0.023 | 1.221 |
Rmeas | 0.165 | 0.028 | 1.459 |
Total number of observations | 5786516 | ||
Number of reflections | 1729501 | 19535 | 125032 |
<I/σ(I)> | 8.58 | 35.37 | 0.97 |
Completeness [%] | 98.3 | 94.1 | 96.4 |
Redundancy | 3.346 | 3.286 | 3.191 |
CC(1/2) | 0.991 | 0.998 | 0.173 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.6 | 292 | 0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME |