5WHP

Crystal structure of the segment, NFGTFS, from the A315T familial variant of the low complexity domain of TDP-43, residues 312-317

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-16
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9791
Spacegroup name	P 1 21 1
Unit cell lengths	15.330, 4.840, 23.570
Unit cell angles	90.00, 96.91, 90.00

Refinement procedure

Resolution	15.220 - 1.000
R-factor	0.0873
Rwork	0.087
R-free	0.09290
Structure solution method	AB INITIO PHASING
RMSD bond length	0.019
RMSD bond angle	1.790
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXD
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	15.220	15.220	1.030
High resolution limit [Å]	1.000	4.250	1.000
Rmerge	0.086	0.068	0.104
Rmeas	0.096	0.083	0.125
Number of reflections	1823	23	57
<I/σ(I)>	14.97	14.57	7.59
Completeness [%]	87.6	62.2	37
Redundancy	4.918	3.348	2.842
CC(1/2)	0.993	0.989	0.980

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	298	100mM sodium acetate 4.6, 200mM ammonium acetate, 30% PEG 4000