5WH1

Apo form of the C-terminal region of human Transcription Factor IIB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	93
Detector technology	CCD
Collection date	2015-07-27
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	108.165, 126.461, 158.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.730 - 3.390
R-factor	0.195
Rwork	0.194
R-free	0.21400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1c9b
RMSD bond length	0.010
RMSD bond angle	1.240
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.730	3.460
High resolution limit [Å]	3.390	3.394
Rmerge	0.239	0.000
Rpim		0.960
Number of reflections	30686	30686
<I/σ(I)>	9.15	1.2
Completeness [%]	99.6	97.4
Redundancy	7.2	7.2
CC(1/2)	0.865	0.546

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	298	100 mM sodium acetate trihydrate pH 4.5, 2 M ammonium sulfate